publications:
- title: "A Zebrafish Model for Studies on Esophageal Epithelial Biology "
  author:
  - name: "Hao Chen"
    link: "http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0143878"
  year: "2015"
  doi: "10.1371/journal.pone.0143878"
  abstract: "Mammalian esophagus exhibits a remarkable change in epithelial structure during the tran- sition from embryo to adult. However, the molecular mechanisms of esophageal epithelial development are not well understood. Zebrafish (Danio rerio), a common model organism for vertebrate development and gene function, has not previously been characterized as a model system for esophageal epithelial development. In this study, we characterized a piece of non-keratinized stratified squamous epithelium similar to human esophageal epi- thelium in the upper digestive tract of developing zebrafish. Under the microscope, this piece was detectable at 5dpf and became stratified at 7dpf. Expression of esophageal epi- thelial marker genes (Krt5, P63, Sox2 and Pax9) was detected by immunohistochemistry and in situ hybridization. Knockdown of P63, a gene known to be critical for esophageal epi- thelium, disrupted the development of this epithelium. With this model system, we found that Pax9 knockdown resulted in loss or disorganization of the squamous epithelium, as well as down-regulation of the differentiation markers Krt4 and Krt5. In summary, we charac- terized a region of stratified squamous epithelium in the zebrafish upper digestive tract which can be used for functional studies of candidate genes involved in esophageal epithe- lial biology. "
  links:
  researchr: "https://researchr.org/publication/a-zebrafish-model-for-studies-on-esophageal-epithelial-biology"
  cites: 1
  citedby: 0
  kind: "article"
  key: "a-zebrafish-model-for-studies-on-esophageal-epithelial-biology"